Concepts in Fluorescence Correlation Spectroscopy
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چکیده
Fluorescence Correlation Spectroscopy (FCS) and related techniques are becoming widely used to measure molecular dynamics in a broad variety of specimens. These techniques provide important quantitative information on dynamics -such as diffusion coefficients, local concentration, binding reactions under equilibrium conditionswith high spatial and temporal resolutions. Also, they are minimal invasive since they work at very low concentrations of fluorescent molecules and do not require the use of high power lasers as Fluorescence Recovery After Photobleaching (FRAP) does, making them very attractive to studies in living specimens.
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